One Knoxville

USL League Two

The club was initially unveiled as Knox Pro Soccer starting as a semi-professional USL League Two club with the goal to eventually move to the professional USL League One in the future. The official name for the team, One Knoxville Sporting Club, was announced on July 15, 2021. The official crest for the team, designed by artist Matthew Wolff, was unveiled on August 19, 2021. Mark McKeever was appointed as the club's first ever head coach on January 13, 2021.

The team made their USL2 debut on May 14, 2022 hosting Asheville City SC, where they were defeated by a score of 2–1 in front of 2200 spectators. They recorded their first victory in their next match on May 17, defeating the Tri-Cities Otters by a score of 1–0. In their inaugural season, they won the South Central Division, with an 11–1–2 record, advancing to the playoffs. After winning their first two playoff rounds, the club was defeated by North Carolina Fusion U23 in the South Conference Finals (league quarterfinals).

USL League One

On October 20, 2022, it was announced that the club would move up to the fully professional level, joining USL League One. The club also announced that all of the team's 15 home games would by played at Regal Stadium, home of the Tennessee Lady Volunteers.

The club announced their first professional signing on December 12, 2022, forward Ilija Ilić, who will also serve as assistant coach.

The club scored its first ever professional win with a 2–1 victory over Lexington SC. Midfielder Jimmie Villalobos scored the team's first ever professional goal.

On April 26, 2025, One Knoxville SC set the record for largest home attendance for a USL1 club across all competitions with 6,378 fans in attendance for a match against FC Tulsa in the opening round of the USL Cup.

On October 25, 2025, One Knoxville SC won its first-ever USL1 regular-season championship, receiving the USL1 Players’ Shield.

On November 16, 2025, One Knoxville SC won their first-ever USL1 league title, marking the club's first major trophy. They defeated Spokane Velocity FC 2–0 in front of a record 7,500 fans at Covenant Health Park.

On April 15, 2026, in the round of 32 of the 2026 U.S. Open Cup, One Knoxville SC upset D.C. United, a team in the MLS, the highest tier of American professional soccer. This marks the 3rd time in history a USL1 team has beaten an MLS team.

Women's team

On March 8, 2025, the club announced the addition of a women's team, which would complete in its inaugural season in the USL W-League beginning that year. Simon Duffy, the women's soccer coach at Carson-Newman University, was hired to lead the new women's soccer team.